Volume 131(2), pp. 137-144
Els D'Hondt (1), Johan Billen (2), Luc Berghman (3), Frans Vandesande (1) and Lutgarde Arckens (1) (email for reprint)
(1) Laboratory of Neuroendocrinology and Immunological Biotechnology, and
(2) Laboratory for Entomology, Katholieke Universiteit Leuven, Naamsestraat 59, B-3000 Leuven, Belgium
(3) Poultry Science Department, Texas A&M University, TX 77843 College Station, USA
Chicken Luteinizing Hormone-Releasing hormone-I and -II are located in distinct fiber terminals in the median eminence of the quail: a light and electron microscopic study
Abstract:
Using highly specific antibodies directed towards the avian luteinizing hormone-releasing hormones-I and II (cLHRH-I and II), we were able to show the presence of both peptides in the median eminence of the quail. In order to examine in more detail the morphological organization of both gonadotropin-releasing hormone systems in the median eminence, the present paper describes the results from a light and ultrastructural immunocytochemical double labeling study. These results demonstrate the presence of cLHRH-I and cLHRH-II in distinct fiber systems in the median eminence of the quail. Moreover, close associations between cLHRH-I or II- immunoreactive neurons and glial cells were observed, suggesting glia1-neuronal interactions.
Key words:LHRH-I, LHRH-II, immunocytochemistry, LM, EM, double staining, bird, median eminence, quail.
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